RESEARCH DOMAINS AT I3TK

OUR RESEARCH DOMAINS

Molecular Biology

MOLECULAR BIOLOGY AND BIOINFORMATICS

Detailed understanding of molecular biology in present day vastly relies on Bioinformatics which includes genomics and proteomics, has contributed to advances in biology by providing tools that handle datasets too large or complex for manual analysis. Examples of some of these tools include assembling of the DNA sequence of entire genomes, gene finding algorithms, microarray expression analysis, molecular system modelling and biomarker discovery from mass spectrum. With extensive facilities of genomics and proteomics, the department of Molecular Biology and Bioinformatics envision the primary goals to understand biological processes at the molecular level. We want to dwell into deep learning, machine learning and also big data analysis & data visualization to study different facets starting from epidemiology to clinical pathology via artificial intelligence (AI).

Mesenchymal Stem Cells (MSCs) are proving to be an important player in regenerative medicine. MSCs have been isolated from various tissue including bone marrow, adipose tissue and umbilical cord. In both pre-clinical and clinical studies, MSCs isolated from Wharton Jelly of the umbilical cord have shown immunomodulatory functions which have been attributed to their immune-privileged state due to their embryonic origin. The therapeutic potential of MSCs have been found, in addition to the cells also in the conditioned media as well as extracellular vesicles secreted by MSCs. At I3T, we are engaged in MSC biology of the Wharton Jelly and are interested in the multiple mode of therapeutic intervention. Understanding the role of MSCs in musculoskeletal processes such as fracture repair, osteoarthritis and muscle and fat related disease condition will be essential to harness the regenerative capacity of MSCs and provide solutions for unmet need of patients.

STEM CELL

Biostatistics and Bioinformatics

Biomarker discovery and identification of interactome, proteome, genome analysis and data set visualization and microarray analysis are the main foci of this department. Integrating bioinformatics at molecular level helps in understanding biological process in an extensive manner and create avenues for therapeutics, development and elucidation of translational clinical research. Statistical validation in order to cross-verify various applicative studies will establish accuracy and precision.

The department of Infectious diseases and Immunology deals with infectious pathogens and their role on public health. Major focuses of this department are to develop novel therapeutics and diagnostics regiments for the treatment of tropical infectious diseases like Malaria, Leishmaniasis and Dengue fever. At I3TK, scientists are involved to identify distinct metabolic pathways of pathogens for drug target. Development of small molecules inhibitors or peptide based inhibitors will be promising candidates to combat the infectious diseases. Moreover in partnership with Johns Hopkins Bloomberg School of Public Health, special attention will be given to develop vaccines against the neglected tropical diseases (NTDs).

INFECTIOUS DISEASES & IMMUNOLOGY

Epidemiology

Epidemiology is the branch of medical science that deals with the factors that determine the presence or absence of diseases. Epidemiology is the study and analysis of the distribution, patterns and determinants of health and disease conditions in defined populations. It is a cornerstone of public health, and shapes policy decisions and evidence-based practice by identifying risk factors for disease and targets for preventive healthcare.

Why do we focus on this : The purpose of epidemiology research is to understand what risk factors are associated with a specific disease, and how disease can be prevented. Epidemiological research helps us to understand how many people have a disease, if those numbers are changing, and how the disease affects our society or our economy. Epidemiological information is used to plan and evaluate strategies to prevent illness and as a guide to the management of patients who are already suffering.

The study of genomics extends the knowledge of gene function from a single gene to the whole genome level. Modern genetics includes complex gene expression studies on a whole genome scale, in the fields of population genetics, disease pathogenesis etc. Our inhouse Ion Torrent Genexus facility is capable of identifying differential RNA expression, single nucleotide polymorphism and analysing soil samples for microbiome identification. While differential RNA expression and single nucleotide polymorphism are directly involved in disease pathogenesis, approaches like microbiome identification are critical to public health in terms of multi drug resistance. Ours is a consolidated approach involving all of the above-mentioned genomic tools in order to uplift the quality of life as a whole.

Genomics and Genetics

Cancer Biology

Cancer is a disease in which some of the body’s cells grow uncontrollably and spread to other parts of the body. Cancer begins when genetic changes interfere with this orderly process. These cells may form a mass called a tumour. A tumour can be cancerous or benign. A cancerous tumour is malignant, meaning it can grow and spread to other parts of the body. A benign tumour means the tumour can grow but will not spread. Different types of cancer are Carcinomas which include prostate cancer, breast cancer, lung cancer, and colorectal cancer, Sarcomas, Leukemias and Lymphomas.

Why does I3T focus on this: I3T is having an Advanced Diagnostic Center. Clinical trials can help researchers find new ways to prevent and detect cancer, and they can also help improve the quality of life for patients during and after treatment.

This department engages in investigation of mammalian cells leveraged as exigent model system to find application in various important aspects like study of various diseases situations (metabolic disorders, cancer, COPD, myocardial infarction etc); biomolecular therapeutics; development of vaccines; elucidation of drug delivery mechanism etc. Study on intracellular signalling pathways and involvement of subcellular organelles unravels yet-unidentified facts on cellular machineries leading to a better mechanistic understanding of specific physiological conditions. The department, with its ‘state-of-the-art’ facility, aspires to achieve, through appropriate translational innovations, significant contributions towards disease management and improvement of public health.

Diabetes is a disease that occurs when your blood glucose, also called blood sugar, is too high. Blood glucose is the main source of energy and comes from the food we eat. Diabetes is a chronic disease that occurs when the pancreas is no longer able to produce insulin, or when the body cannot make good use of the insulin it produces. Insulin, a hormone made by the pancreas, helps glucose from food get into our cells to be used for energy. Sometimes our body doesn’t make enough—or any—insulin or doesn’t use insulin well. Glucose then stays in our blood and doesn’t reach our cells. There are three main types of diabetes – type 1, type 2 and gestational.

Scientists at I3TK will address the issue by developing novel therapeutic intervention to treat diabetic conservancies. Pathophysiological models will be established ‘in-vitro’ and ‘in-vivo’ and efficacy of strategies will be investigated. Cost-effective disease-associated financial burden of community.

Cell Biology and Metabolic Disorder

Virology

Virology is the branch of microbiology that deals with the study of viruses (as well as various virus-like particles), their characteristics, classification, as well as the relationship with their respective hosts. I3T is a public health institution and wants to spread the outreach so that we will work on all public health issues under a single roof. I3T has advanced instruments, ultra modern infrastructure and scientists. We are willing to work on Infectious, communicable and non-communicable diseases such as Dengue, Malaria, Hepatitis, Cancer, Diabetes and Virology.

The overall study of neuroanatomy and neurophysiology is generally known as neurobiology. Our research interest primarily involves pathological conditions like Autism, Parkinson’s disease, Alzheimer’s disease. Although these are quite sought-after research topics in the field of neurobiology, biomarker development needs more focus. Modern technological advances in transcriptomics, proteomics, and metabolomics offer many crucial insights into the disease mechanism, as well as opportunities for the development of novel therapeutics that can reverse and/or halt neurodegeneration. I3tK laboratory is equipped with BSL-2 cell culture facility and state of the art facilities supporting the on-going projects on biomarker development.

Neurobiology

Food Technology

I3t in its constant pursuit of decent public health is involved in Food making and processing. Current efforts of I3T include fortified foods, organic energy drinks and supplements and nutraceuticals.