{"id":12848,"date":"2022-12-07T12:58:02","date_gmt":"2022-12-07T07:28:02","guid":{"rendered":"https:\/\/i3tk.org\/demo2\/?p=12848"},"modified":"2022-12-07T14:39:43","modified_gmt":"2022-12-07T09:09:43","slug":"sars-cov-2","status":"publish","type":"post","link":"https:\/\/i3tk.org\/demo2\/blog\/sars-cov-2\/","title":{"rendered":"SARS-Cov-2 and Testing Methods"},"content":{"rendered":"<p>At the end of the 2019, a virus named Severe Acute Respiratory Syndrome Coronavirus (<a href=\"https:\/\/en.wikipedia.org\/wiki\/SARS-CoV-2\">SARS- Cov-2)<\/a> detected in Wuhan, China. Until November 2021, diagnosis of more than 247 million people\u00a0 and death of 5.01 million people patients\u00a0 due to Cov-2 virus.<br \/>\nAlthough vaccines and treatments are going on , side by side diagnosis of Covid-19 virus in several methods are under process. There are many serological and nucleic acid amplification test are<br \/>\ndone, but some specific test are also there for accurate result.<\/p>\n<h2>The various Tests are as follows:<\/h2>\n<p><strong>ddRT-PCR-<\/strong><\/p>\n<ul>\n<li>This technology is the third-generation RT-PCR method and not requires the standard curve line to achieve absolute quantitative detection of DNA.<\/li>\n<li>It greatly improves the accuracy of diagnosis, especially for the detection of samples with low copy SARS-CoV by using droplet technology.<strong>RT-LAMP<\/strong>&#8211;<\/li>\n<\/ul>\n<p><strong>RT-LAMP-<\/strong><\/p>\n<ul>\n<li>\u00a0LAMP is a new isothermal nucleic acid amplification method with great efficiency.<\/li>\n<li>The LAMP technology does not need high-priced equipment and reagents with the high advancement of saving time.<\/li>\n<li>It\u2019s very easy to observe the results for using the Gel Electrophoresis method. So, this is a\u00a0 cost- effective method.<\/li>\n<li>\u00a0It has exponential amplification feature and six particular target sequences diagnosed by four separator primers.<\/li>\n<li>Within one hour and under constant temperature (60\u201365\u00b0C), the templet can be amplified to 10 9 ~10 10 \u00a0 times.<\/li>\n<li>The results of the LAMP can be easily judged by the turbidity or color of the solution.<\/li>\n<\/ul>\n<p><strong>CRISPRE<\/strong>&#8211;<\/p>\n<ul>\n<li>The test detection method using enzyme Cas13a\/C2c2 based on clustered regularly interspaced short palindromic repeats is a fast, cheap and highly sensitive diagnostics tool.<\/li>\n<li>\u00a0The Cas13a detects the target RNA sequence and become active state(Under the guidance CRISPR RNA).When the target RNA sequence is cut, it\u00a0 emits a fluorescent signal.<\/li>\n<li>\u00a0This method is easy to operate .It can test quickly without the need for professionals, reducing the shortage of professionals in the large scale fight against COVID-19.<\/li>\n<li>Beside the methods, other RNA-based method for the detection of SARS-CoV-2 is microarray. It is a rapid and high- throughput method for diagnosis of COVID-19. cDNA is first<br \/>\nproduced by coronavirus RNA template. Then the targets are hybridized to the probes. Remove free DNAs\u00a0 by washing the solution and finally probes identify SARS-CoV-2 RNA.<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<h4>Reference: <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8761393\/#jcla2 4178-bib-0014\">Check here<\/a><\/h4>\n<p>&nbsp;<\/p>\n<p><img decoding=\"async\" class=\"lazyload aligncenter wp-image-12852 size-large\" src=\"https:\/\/i3tk.org\/demo2\/wp-content\/uploads\/2022\/12\/SARS-Cov-2-1024x576.png\" data-orig-src=\"https:\/\/i3tk.org\/demo2\/wp-content\/uploads\/2022\/12\/SARS-Cov-2-1024x576.png\" alt=\"Rt Lamp &amp; CRISPR testing method\" width=\"1024\" height=\"576\" srcset=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%27576%27%20viewBox%3D%270%200%201024%20576%27%3E%3Crect%20width%3D%271024%27%20height%3D%27576%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-srcset=\"https:\/\/i3tk.org\/demo2\/wp-content\/uploads\/2022\/12\/SARS-Cov-2-200x113.png 200w, https:\/\/i3tk.org\/demo2\/wp-content\/uploads\/2022\/12\/SARS-Cov-2-300x169.png 300w, https:\/\/i3tk.org\/demo2\/wp-content\/uploads\/2022\/12\/SARS-Cov-2-400x225.png 400w, https:\/\/i3tk.org\/demo2\/wp-content\/uploads\/2022\/12\/SARS-Cov-2-600x338.png 600w, https:\/\/i3tk.org\/demo2\/wp-content\/uploads\/2022\/12\/SARS-Cov-2-768x432.png 768w, https:\/\/i3tk.org\/demo2\/wp-content\/uploads\/2022\/12\/SARS-Cov-2-800x450.png 800w, https:\/\/i3tk.org\/demo2\/wp-content\/uploads\/2022\/12\/SARS-Cov-2-1024x576.png 1024w, https:\/\/i3tk.org\/demo2\/wp-content\/uploads\/2022\/12\/SARS-Cov-2-1200x675.png 1200w, https:\/\/i3tk.org\/demo2\/wp-content\/uploads\/2022\/12\/SARS-Cov-2-1536x864.png 1536w\" data-sizes=\"auto\" data-orig-sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<p>At the end of the 2019, a virus named Severe [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":12856,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[41],"tags":[159,156,160,161,158,157],"class_list":["post-12848","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-blog","tag-cov-2","tag-covid","tag-crispr","tag-rt-lamp","tag-sars","tag-testing"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v18.8 (Yoast SEO v27.7) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>SARS-Cov-2<\/title>\n<meta name=\"description\" content=\"The SARS-CoV-2 virus causes an infectious disease Coronavirus disease (COVID-19). 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