{"id":12749,"date":"2022-11-05T11:50:53","date_gmt":"2022-11-05T06:20:53","guid":{"rendered":"https:\/\/i3tk.org\/demo2\/?page_id=12749"},"modified":"2022-11-05T12:07:17","modified_gmt":"2022-11-05T06:37:17","slug":"instrument-infrastructure","status":"publish","type":"page","link":"https:\/\/i3tk.org\/demo2\/instrument-infrastructure\/","title":{"rendered":"Instrument &#038; Infrastructure"},"content":{"rendered":"<style type='text\/css' media='all'>#tss-container-2326178056.tss-wrapper .tss-layout10 .item-content-wrapper,\n\t\t\t\t\t#tss-container-2326178056.tss-wrapper .tss-isotope3 .item-content-wrapper,\n\t\t\t\t\t#tss-container-2326178056.tss-wrapper .tss-isotope5 .item-content-wrapper,\n\t\t\t\t\t#tss-container-2326178056.tss-wrapper .tss-carousel10 .item-content-wrapper{background: transparent;}<\/style><div class='rt-container-fluid tss-wrapper ' id='tss-container-2326178056'  data-layout='carousel1' data-desktop-col='1'  data-tab-col='1'  data-mobile-col='1'><div data-title='Loading ...' class='rt-row tss-carousel1 tss-even tss-pre-loader'><div class=\"carousel-wrapper\"><div  class='tss-carousel swiper'\n\t\t\t\t\t\t\t\t\t\tdata-loop='false'\n\t\t\t\t\t\t\t\t\t\tdata-items-desktop='1'\n\t\t\t\t\t\t\t\t\t\tdata-items-tab='1'\n\t\t\t\t\t\t\t\t\t\tdata-items-mobile='1'\n\t\t\t\t\t\t\t\t\t\tdata-autoplay='true'\n\t\t\t\t\t\t\t\t\t\tdata-autoplay-timeout='5000'\n\t\t\t\t\t\t\t\t\t\tdata-autoplay-hover-pause='false'\n\t\t\t\t\t\t\t\t\t\tdata-dots='true'\n\t\t\t\t\t\t\t\t\t\tdata-nav='false'\n\t\t\t\t\t\t\t\t\t\tdata-lazy-load='false'\n\t\t\t\t\t\t\t\t\t\tdata-auto-height='false'\n\t\t\t\t\t\t\t\t\t\tdata-smart-speed='2000'\n\t\t\t\t\t\t\t\t\t\t><div class=\"swiper-wrapper\"><div class='rt-col-md-12 rt-col-sm-12 rt-col-xs-12 even-grid-item tss-grid-item slide-item swiper-slide even-grid-item default-margin'><div class=\"single-item-wrapper\"><div class=\"tss-meta-info tss-left\"><div class='profile-img-wrapper'><img alt='ZYBIO NUCLEIC ACID AUTO EXTRACTOR S\/N nu:' class='rt-responsive-img' src='https:\/\/i3tk.org\/demo2\/wp-content\/uploads\/2022\/11\/testimonial_8-150x150.png' \/><\/div><h3 class='author-name'>ZYBIO NUCLEIC ACID AUTO EXTRACTOR S\/N nu:<\/h3><div class=\"rating-wrapper\"><span data-star='1' class='star-1 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='2' class='star-2 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='3' class='star-3 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='4' class='star-4 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='5' class='star-5 dashicons dashicons-star-empty' aria-hidden='true'><\/span><\/div><\/div><div class=\"item-content-wrapper tss-right\"><div class='item-content'>\u2b9a\tMACHINE SPECIFICATION:\r\n\u25cf\tAnalytical method Magnetic bead method\r\n\u25cf\tThroughput 1-32 samples\r\n\u25cf\tProcess volume 30 \u2013 1000 \u00b5l\r\n\u25cf\t For virus detected take time 9 mins.\r\n<\/div><\/div><\/div><\/div><div class='rt-col-md-12 rt-col-sm-12 rt-col-xs-12 even-grid-item tss-grid-item slide-item swiper-slide even-grid-item default-margin'><div class=\"single-item-wrapper\"><div class=\"tss-meta-info tss-left\"><div class='profile-img-wrapper'><img alt='BIO-RAD CFX 96 TOUCH THERMAL CYCLER REAL TIME PCR DETECTION SYSTEM S\/N nu: 787BR10301' class='rt-responsive-img' src='https:\/\/i3tk.org\/demo2\/wp-content\/uploads\/2022\/11\/testimonial_7-150x150.png' \/><\/div><h3 class='author-name'>BIO-RAD CFX 96 TOUCH THERMAL CYCLER REAL TIME PCR DETECTION SYSTEM S\/N nu: 787BR10301<\/h3><div class=\"rating-wrapper\"><span data-star='1' class='star-1 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='2' class='star-2 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='3' class='star-3 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='4' class='star-4 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='5' class='star-5 dashicons dashicons-star-empty' aria-hidden='true'><\/span><\/div><\/div><div class=\"item-content-wrapper tss-right\"><div class='item-content'>\u2b9a\tMACHINE SPECIFICATION:\r\n\u25cf\tThe CFX96 touch system is a powerful, precise, and flexible real time PCR detection.\r\n\u25cf\tSamples capacity 96 wells at once.\r\n\u25cf\tSample size 1 \u2013 50\u00b5l (10 \u2013 25 recommended)\r\n<\/div><\/div><\/div><\/div><div class='rt-col-md-12 rt-col-sm-12 rt-col-xs-12 even-grid-item tss-grid-item slide-item swiper-slide even-grid-item default-margin'><div class=\"single-item-wrapper\"><div class=\"tss-meta-info tss-left\"><div class='profile-img-wrapper'><img alt='URISCAN OPTIMA (URINE ANALYSER) S\/N nu: UD-60911674' class='rt-responsive-img' src='https:\/\/i3tk.org\/demo2\/wp-content\/uploads\/2022\/11\/testimonial_6-150x150.png' \/><\/div><h3 class='author-name'>URISCAN OPTIMA (URINE ANALYSER) S\/N nu: UD-60911674<\/h3><div class=\"rating-wrapper\"><span data-star='1' class='star-1 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='2' class='star-2 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='3' class='star-3 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='4' class='star-4 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='5' class='star-5 dashicons dashicons-star-empty' aria-hidden='true'><\/span><\/div><\/div><div class=\"item-content-wrapper tss-right\"><div class='item-content'>\u2b9a\tMACHINE SPECIFICATION:\r\n\u25cf\tIt\u2019s a semi-automated urine analyzer.\r\n\u25cf\tMemory capacity 1,000 tests.\r\n\u25cf\tAnalyze method Reflectance photometers.\r\n\u25cf\tTest capacity: Routine mode 36 samples\/hour.\r\n                             Quick mode 300 samples\/hour.\r\n\u25cf\tMeasureable time: Routine mode 100 seconds.\r\n                                       Quick mode  6 seconds.\r\n\u25cf\tMemory capacity 2000 patients data.\r\n<\/div><\/div><\/div><\/div><div class='rt-col-md-12 rt-col-sm-12 rt-col-xs-12 even-grid-item tss-grid-item slide-item swiper-slide even-grid-item default-margin'><div class=\"single-item-wrapper\"><div class=\"tss-meta-info tss-left\"><div class='profile-img-wrapper'><img alt='MIX-RATE X20 ERYTHROCYTE SEDIMENTATION RATE  S\/N nu: 19-12040' class='rt-responsive-img' src='https:\/\/i3tk.org\/demo2\/wp-content\/uploads\/2022\/11\/testimonial_5-150x150.png' \/><\/div><h3 class='author-name'>MIX-RATE X20 ERYTHROCYTE SEDIMENTATION RATE  S\/N nu: 19-12040<\/h3><div class=\"rating-wrapper\"><span data-star='1' class='star-1 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='2' class='star-2 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='3' class='star-3 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='4' class='star-4 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='5' class='star-5 dashicons dashicons-star-empty' aria-hidden='true'><\/span><\/div><\/div><div class=\"item-content-wrapper tss-right\"><div class='item-content'>\u2b9a\tMACHINE SPECIFICATION:\r\n\u25cf\tHolding up to 20 samples with capability of performing up to 80 ESR tests per hour.\r\n\u25cf\tReading time 15 mins.\r\n\u25cf\tExcellent correlation with the manual Westgren method.\r\n<\/div><\/div><\/div><\/div><div class='rt-col-md-12 rt-col-sm-12 rt-col-xs-12 even-grid-item tss-grid-item slide-item swiper-slide even-grid-item default-margin'><div class=\"single-item-wrapper\"><div class=\"tss-meta-info tss-left\"><div class='profile-img-wrapper'><img alt='FULL AUTOMATED UniCel DXH-800 CELL COUNTER (HAEMATOLOGY ANALYZER)  S\/N nu: BD03129' class='rt-responsive-img' src='https:\/\/i3tk.org\/demo2\/wp-content\/uploads\/2022\/11\/testimonial_4-150x150.png' \/><\/div><h3 class='author-name'>FULL AUTOMATED UniCel DXH-800 CELL COUNTER (HAEMATOLOGY ANALYZER)  S\/N nu: BD03129<\/h3><div class=\"rating-wrapper\"><span data-star='1' class='star-1 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='2' class='star-2 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='3' class='star-3 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='4' class='star-4 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='5' class='star-5 dashicons dashicons-star-empty' aria-hidden='true'><\/span><\/div><\/div><div class=\"item-content-wrapper tss-right\"><div class='item-content'>\u2b9a\tMACHINE SPECIFIACTION:\r\n\u25cf\tThe UniCel DXH-800 analyzer is quantitative, automated haematology analyzer for in vitro diagnostics use. \r\n\u25cf\tAnalyzing method Coulter method.\r\n\u25cf\tThroughput up to 100 samples\/hour\r\n\u25cf\tSamples aspiration volume 165 \u00b5L. \r\n<\/div><\/div><\/div><\/div><div class='rt-col-md-12 rt-col-sm-12 rt-col-xs-12 even-grid-item tss-grid-item slide-item swiper-slide even-grid-item default-margin'><div class=\"single-item-wrapper\"><div class=\"tss-meta-info tss-left\"><div class='profile-img-wrapper'><img alt='SEMI-AUTOMATED ST-200 PLUS ELECTROLYTE ANALYZER  S\/N nu: ST-7250' class='rt-responsive-img' src='https:\/\/i3tk.org\/demo2\/wp-content\/uploads\/2022\/11\/testimonial_3-150x150.png' \/><\/div><h3 class='author-name'>SEMI-AUTOMATED ST-200 PLUS ELECTROLYTE ANALYZER  S\/N nu: ST-7250<\/h3><div class=\"rating-wrapper\"><span data-star='1' class='star-1 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='2' class='star-2 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='3' class='star-3 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='4' class='star-4 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='5' class='star-5 dashicons dashicons-star-empty' aria-hidden='true'><\/span><\/div><\/div><div class=\"item-content-wrapper tss-right\"><div class='item-content'>\u2b9a\tMACHINE SPECIFICATION:\r\n\u25cf\tThe ST-200 plus electrolyte analyzer is a Semi-automated analyzer are specifically designed to meet the needs to small to medium sized laboratories by inventing and developing the latest technology. Extremely low cost per test. \r\n\u25cf\tSample volume 150 \u00b5L.\r\n\u25cf\tMeasuring time 60 sec.\r\n\u25cf\tSample type Whole blood, Serum, Plasma, CSF, Diluted urine(1:5).\r\n\u25cf\tParameters included Na, K, Ca, Cl, Li, pH.\r\n\u25cf\tThroughput 55tests per hour.\r\n\u25cf\tAnalytical principle Direct measurement with ISE.\r\n<\/div><\/div><\/div><\/div><div class='rt-col-md-12 rt-col-sm-12 rt-col-xs-12 even-grid-item tss-grid-item slide-item swiper-slide even-grid-item default-margin'><div class=\"single-item-wrapper\"><div class=\"tss-meta-info tss-left\"><div class='profile-img-wrapper'><img alt='FULL AUTOMATED IMMUNO ASSAY ANALYZER ACCESS-2 S\/N nu: 572200' class='rt-responsive-img' src='https:\/\/i3tk.org\/demo2\/wp-content\/uploads\/2022\/11\/testimonial_2-150x150.png' \/><\/div><h3 class='author-name'>FULL AUTOMATED IMMUNO ASSAY ANALYZER ACCESS-2 S\/N nu: 572200<\/h3><div class=\"rating-wrapper\"><span data-star='1' class='star-1 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='2' class='star-2 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='3' class='star-3 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='4' class='star-4 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='5' class='star-5 dashicons dashicons-star-empty' aria-hidden='true'><\/span><\/div><\/div><div class=\"item-content-wrapper tss-right\"><div class='item-content'>\u2b9a\tMACHINE SPECIFICATION:\r\n\u25cf\tThe Access 2 contains the robustness of a reference laboratory immunoassay analyzer in the convenient size, providing greater control over performance, reliability and speed without sacrificing valuable laboratory space.\r\n\u25cf\tThroughput of up to 100 tests per hour.\r\n\u25cf\tLoading capacity 60 samples at once.\r\n\u25cf\tRandom access for running any combination of samples any time.\r\n\u25cf\tRapid kinetics for improved turnaround time.\r\n\u25cf\tStat and automatic reflex testing\r\n\u25cf\tOnboard refrigerated storage of up to 24 hours.\r\n\u25cf\t Utilizes Proven chemiluminescent and magnetic particle separation technology.\r\n\u25cf\tSample type Serum, Plasma, Urine, Amniotic fluid, Whole blood.\r\n\u25cf\tSample volume 10-100 \u03bcl.\r\n<\/div><\/div><\/div><\/div><div class='rt-col-md-12 rt-col-sm-12 rt-col-xs-12 even-grid-item tss-grid-item slide-item swiper-slide even-grid-item default-margin'><div class=\"single-item-wrapper\"><div class=\"tss-meta-info tss-left\"><div class='profile-img-wrapper'><img alt='FULL  AUTOMATED BIOCHEMISTRY ANALYZER BECKMEN COULTER AU-480' class='rt-responsive-img' src='https:\/\/i3tk.org\/demo2\/wp-content\/uploads\/2022\/11\/testimonial_1.jpg-150x150.png' \/><\/div><h3 class='author-name'>FULL  AUTOMATED BIOCHEMISTRY ANALYZER BECKMEN COULTER AU-480<\/h3><div class=\"rating-wrapper\"><span data-star='1' class='star-1 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='2' class='star-2 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='3' class='star-3 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='4' class='star-4 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='5' class='star-5 dashicons dashicons-star-empty' aria-hidden='true'><\/span><\/div><\/div><div class=\"item-content-wrapper tss-right\"><div class='item-content'>\u2b9a MACHINE SPECIFICATION:\r\n\u25cf The AU480 is the ideal primary chemistry analyzer to small to medium sized hospitals and laboratories, or as a dedicated specialty chemical or stat analyzer for larger institutions.\r\n\u25cf Analytical principal Spectrophotometry and potentiometry.\r\n\u25cf Analytical methods Colorimetry, Turbidimetry, Latex agglutination, Homogeneous EIA, Indirect ISE. \r\n\u25cf Its throughput is up to 400 photometric test per hour(up to 800 with electrolytes).\r\n\u25cf Increased onboard testing, reduced sample volume and user friendly operation.\r\n\u25cf Sample volume need 1 \u2013 25 \u03bcl in 0 .1 \u03bcl steps.\r\n\u25cf Samples integrity Lipaemia, Haemolysis and Icterus analysis.\r\n\u25cf Samples types Serum, Plasma, Urine, other.\r\n<\/div><\/div><\/div><\/div><div class='rt-col-md-12 rt-col-sm-12 rt-col-xs-12 even-grid-item tss-grid-item slide-item swiper-slide even-grid-item default-margin'><div class=\"single-item-wrapper\"><div class=\"tss-meta-info tss-left\"><div class='profile-img-wrapper'><img alt='The high speed advanced centrifuge' class='rt-responsive-img' src='https:\/\/i3tk.org\/demo2\/wp-content\/uploads\/2022\/11\/img_16-150x150.jpg' \/><\/div><h3 class='author-name'>The high speed advanced centrifuge<\/h3><div class=\"rating-wrapper\"><span data-star='1' class='star-1 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='2' class='star-2 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='3' class='star-3 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='4' class='star-4 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='5' class='star-5 dashicons dashicons-star-empty' aria-hidden='true'><\/span><\/div><\/div><div class=\"item-content-wrapper tss-right\"><div class='item-content'>A centrifuge is a mechanical or electromechanical device used to separate various components of a liquid. It achieves that by simulating a very high gravity environment inside the tubes by producing centrifugal forces by spinning very fast.\r\n\r\n<strong>At I3T our laboratory is equipped with Centrifuge 5804R by eppendorf,<\/strong> which is an advanced high-speed benchtop centrifuge. Centrifuge 5804\/5804 R is suitable for medium-throughput molecular biology applications. It handles all common tube formats from 0.2\u202fmL to 250 mL as well as plates, offering a high versatility acknowledgement to a broad range of rotors and adapters.<\/div><\/div><\/div><\/div><div class='rt-col-md-12 rt-col-sm-12 rt-col-xs-12 even-grid-item tss-grid-item slide-item swiper-slide even-grid-item default-margin'><div class=\"single-item-wrapper\"><div class=\"tss-meta-info tss-left\"><div class='profile-img-wrapper'><img alt='Advanced Imaging system' class='rt-responsive-img' src='https:\/\/i3tk.org\/demo2\/wp-content\/uploads\/2022\/11\/img_15-150x150.jpg' \/><\/div><h3 class='author-name'>Advanced Imaging system<\/h3><div class=\"rating-wrapper\"><span data-star='1' class='star-1 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='2' class='star-2 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='3' class='star-3 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='4' class='star-4 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='5' class='star-5 dashicons dashicons-star-empty' aria-hidden='true'><\/span><\/div><\/div><div class=\"item-content-wrapper tss-right\"><div class='item-content'><strong>At I3T our cell culture laboratory is equipped with EVOS M5000 imaging system by invitrogen. Thermo Fisher Scientific. <\/strong>\r\n\r\nThe EVOS M5000 microscope is a workhorse for cell imaging labs across a broad range of applications and imaging requirements. It is a fully integrated digital inverted bench top microscope with a high-resolution CMOS monochrome camera capable of four-color fluorescence, transmitted light, and color applications, the EVOS M5000 delivers publication-ready images in seconds with minimal personal contact. With a five-position objective turret and the ability to acquire images in four fluorescence channels, transmitted light phase-contrast, and color, the optical system can be easily configured.<\/div><\/div><\/div><\/div><div class='rt-col-md-12 rt-col-sm-12 rt-col-xs-12 even-grid-item tss-grid-item slide-item swiper-slide even-grid-item default-margin'><div class=\"single-item-wrapper\"><div class=\"tss-meta-info tss-left\"><div class='profile-img-wrapper'><img alt='Phase contrast microscope' class='rt-responsive-img' src='https:\/\/i3tk.org\/demo2\/wp-content\/uploads\/2022\/11\/img_14-150x150.jpg' \/><\/div><h3 class='author-name'>Phase contrast microscope<\/h3><div class=\"rating-wrapper\"><span data-star='1' class='star-1 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='2' class='star-2 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='3' class='star-3 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='4' class='star-4 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='5' class='star-5 dashicons dashicons-star-empty' aria-hidden='true'><\/span><\/div><\/div><div class=\"item-content-wrapper tss-right\"><div class='item-content'>Phase contrast is a light microscopy technique used to enhance the contrast of images of transparent and colourless specimens. It enables visualisation of cells and cell components that would be difficult to see using an ordinary light microscope. As phase contrast microscopy does not require cells to be killed, fixed or stained, the technique enables living cells, usually in culture, to be visualised in their natural state. This means biological processes can be seen and recorded at high contrast and specimen detail can be observed. Fluorescence staining can be used in combination with phase contrast to further improve the visualisation of samples.\r\n\r\n<strong>At I3T our cell culture laboratory has a Metzer \u2013 M trinocular tissue culture microscope - 6000 TTCM (star), which is an inverted phase contrast microscope, allows us to examine the culture of live cells.<\/strong><\/div><\/div><\/div><\/div><div class='rt-col-md-12 rt-col-sm-12 rt-col-xs-12 even-grid-item tss-grid-item slide-item swiper-slide even-grid-item default-margin'><div class=\"single-item-wrapper\"><div class=\"tss-meta-info tss-left\"><div class='profile-img-wrapper'><img alt='CO2 Incubator' class='rt-responsive-img' src='https:\/\/i3tk.org\/demo2\/wp-content\/uploads\/2022\/11\/img_13-150x150.jpg' \/><\/div><h3 class='author-name'>CO2 Incubator<\/h3><div class=\"rating-wrapper\"><span data-star='1' class='star-1 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='2' class='star-2 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='3' class='star-3 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='4' class='star-4 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='5' class='star-5 dashicons dashicons-star-empty' aria-hidden='true'><\/span><\/div><\/div><div class=\"item-content-wrapper tss-right\"><div class='item-content'>A CO\u2082 incubator plays an important role in maintaining a cell culture\u2019s sterility, temperature, humidity, and pH\u2014parameters that are to maintain the culture\u2019s viability. CO<sub>2 <\/sub>incubator is an essential requirement for growing mammalian cells in an artificial environment.\r\n\r\nCell culture is one of the major tools used in cellular and molecular biology, providing excellent model systems for studying the normal physiology and biochemistry of cells (e.g., metabolic studies, aging), the effects of drugs and toxic compounds on the cells and mutagenesis and carcinogenesis. It is also used in drug screening and development and large scale manufacturing of biological compounds (e.g., vaccines, therapeutic proteins).\r\n\r\nOur cell culture laboratory facilitated by the <strong>Thermo Scientific\u2122 Heracell\u2122 VIOS 160i CO2 Incubator, which <\/strong>is designed to maintain a constant temperature and high humidity for the growth of tissue culture cells under a CO<sub>2<\/sub> atmosphere.\r\n\r\nThe conditions we generally maintained at this facility are 5% CO<sub>2<\/sub> , pH 7.4, 98.6\u00b0F (37\u00b0C) temperature, and &gt; 90% relative humidity.<\/div><\/div><\/div><\/div><div class='rt-col-md-12 rt-col-sm-12 rt-col-xs-12 even-grid-item tss-grid-item slide-item swiper-slide even-grid-item default-margin'><div class=\"single-item-wrapper\"><div class=\"tss-meta-info tss-left\"><div class='profile-img-wrapper'><img alt='Biosafety cabinet (BSC)' class='rt-responsive-img' src='https:\/\/i3tk.org\/demo2\/wp-content\/uploads\/2022\/11\/img_12-150x150.jpg' \/><\/div><h3 class='author-name'>Biosafety cabinet (BSC)<\/h3><div class=\"rating-wrapper\"><span data-star='1' class='star-1 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='2' class='star-2 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='3' class='star-3 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='4' class='star-4 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='5' class='star-5 dashicons dashicons-star-empty' aria-hidden='true'><\/span><\/div><\/div><div class=\"item-content-wrapper tss-right\"><div class='item-content'>The term biosafety cabinet have been widely used to describe a variety of containment devices equipped with HEPA filter(s), designed to provide personnel or both personnel and product protection from biohazardous materials. The terms should only be applied to those devices that meet the requirements of Class I, II, or III specifications, based on their construction, airflow velocities and patterns, and their exhaust systems.\r\n\r\nA feature unique to BSC Class II cabinets is a vertical laminar (unidirectional) HEPA-filtered air stream that descends downward from the interior of the cabinet. This continuously flushes the cabinet interior of airborne contaminants and protects samples being handled within the cabinet from contamination and is known as the downflow.\r\n\r\n<strong>Our cell culture laboratory equipped with Thermo Scientific\u2122 1300 Series Class II, Type A2.<\/strong>\r\n\r\nThe Class II Type A2 that has a negative pressure surrounding the positively pressurized contaminated plenum. In case there is a leakage on the positive plenum, the leaking aerosol will be pulled by the negative pressure back to the positive plenum, and it will not leak out. In the A2 cabinet, about 70% of air from the positive plenum is recirculated as downflow, and the remaining 30% is discharged to the lab through the exhaust filter.<\/div><\/div><\/div><\/div><div class='rt-col-md-12 rt-col-sm-12 rt-col-xs-12 even-grid-item tss-grid-item slide-item swiper-slide even-grid-item default-margin'><div class=\"single-item-wrapper\"><div class=\"tss-meta-info tss-left\"><div class='profile-img-wrapper'><img alt='Biosafety level 2(BSL 2)' class='rt-responsive-img' src='https:\/\/i3tk.org\/demo2\/wp-content\/uploads\/2022\/11\/img_11-1-150x150.jpg' \/><\/div><h3 class='author-name'>Biosafety level 2(BSL 2)<\/h3><div class=\"rating-wrapper\"><span data-star='1' class='star-1 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='2' class='star-2 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='3' class='star-3 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='4' class='star-4 dashicons dashicons-star-empty' aria-hidden='true'><\/span><span data-star='5' class='star-5 dashicons dashicons-star-empty' aria-hidden='true'><\/span><\/div><\/div><div class=\"item-content-wrapper tss-right\"><div class='item-content'>Biosafety level or BSL is a set of biocontainment precautions required to keep the biohazards under control.\r\n\r\nBiosafety Level 2 (BSL-2) is suitable for work involving agents that pose moderate hazards to personnel and the environment. Generally, agents handled at this level have vaccines or therapies readily available and are not an inhalation exposure risk under routine handling conditions.\r\n\r\nBecause of these features, a standard lab space with procedures and equipment in place that minimize the potential for personnel to have dermal or mucous membrane contact with contaminated materials is the basis of this biosafety level.\r\n\r\nContainment emphasis is on safe sharps handling techniques and minimizing the spread of contamination to lab surfaces, including the use of a biological safety cabinet (BSC) for aerosol- generating procedures.\r\n\r\nAlthough newly constructed BSL-2 labs are expected to have inward airflow, escape of contaminants outside of a BSL-2 lab will most likely result from a break in defined work practices.\r\n\r\n<strong>At I3T we have advanced BSL 2 facilities which <\/strong><strong>suitable for work involving agents of moderate potential hazard to personnel and the environment.<\/strong><\/div><\/div><\/div><\/div><\/div><div class=\"swiper-pagination\"><\/div><\/div><\/div><div class=\"rt-loading-overlay\"><\/div><div class=\"rt-loading rt-ball-clip-rotate\"><div><\/div><\/div><\/div><\/div>\n<style>\n .dashicons-star-empty{\n    display : none; 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